Our aligned electrospun Mimetix scaffolds allow:
- Differentiation of more than 80% of oligodendrocyte precursors into mature oligodendrocytes in 14 days.
- Production of sheath lengths comparable to in vivo situation within 7 days by 90% of the oligodendrocytes.
- Physical guidance and support for Schwann cells.
EXAMPLE APPLICATIONS
• MYELINATION BY OLIGODENDROCYTES ON MIMETIX ALIGNED FIBER 96-WELL PLATES
Single cell image (left) shows a 6-week old oligodendrocyte from neonatal rat. Fibers are in
blue, with cell nucleus in white and body in green. Red tendrils coiling around the fibers are Myelin Basic Protein (MBP), as a marker of myelin sheaths.
(The whole cell stretches well over 200um) Image at right shows a large area of a well (~3-4mm 2) showing nuclei (blue) and MBP (red). This is more or less
what you see if you look at a well with low power lens using fluorescence microscopy. (Images courtesy of Daryan Chitsaz, Kennedy Lab, McGill University,
Montreal, Canada)
• USE OF RHODAMINE TO VISUALIZE FIBERS
Our Mimetix Aligned Fibre 384-well scaffolds are available
with rhodamine or
without rhodamine incorporated in the fibers. Rhodamine can help with visualizing the system, as shown below
Representative immunocytochemistry images of MBP+ myelinating oligodendrocytes (green), AND rhodamine+ microfibers
(purple) at day 10. Reproduced under
Creative Commons CC-BY
license from Ellitt et al 2019
GOLGI OUTPOSTS
Oligodendrocyte cultured on Mimetix 3D microfibers for 14 days and stained against tubulin (image courtesy of
Meng-Meng Fu, Stanford University Medical School)
Scientists at Stanford University Medical School have used Mimetix® aligned 3D cell culture scaffolds to understand the architecture of the myelin
sheaths which insulate neuronal axons, so they can transmit electrical impulses quickly and efficiently.
Oligodendrocyte cells in the central nervous system can contact up to 50 axon segments per cell to form such myelin sheaths. Meng-Meng Fu of Stanford University and
co-authors cultured oligodendrocytes in 3D in vitro on Mimetix® aligned 3D cell culture scaffolds, showing that they form satellite organelles called
“Golgi Outposts”. These organize the complex architecture of microtubules to support the branching structure of myelin sheaths.
The research identifies a specific marker for Golgi outposts – TPPP (Tubulin Polymerization Promoting Protein) that was used to purify this
organelle and characterize its proteome. Tppp knockout oligodendrocytes have shorter sheaths in vitro; while Tppp knockout mice have hypomyelination and motor coordination
defects in vivo.
Meng-Meng Fu commented: "The Mimetix 3D microfibers from AMSBIO have allowed us to understand the 3D shape of oligodendrocytes. This is an invaluable
tool that yielded information that we would not have been able to acquire using traditional 2D cell culture platforms. Using 3D microfibers to culture mature oligodendrocytes
for up to 2 weeks, we were able to measure myelin sheath length and number of sheaths per cell. In addition, we showed in
our paper that these data were consistent with in
vivo measurements taken from individual oligodendrocytes in the cortex."
ORDERING DETAILS
Full manual (Mimetix Scaffolds)
RELATED PRODUCTS
PUBLISHED CITATIONS
First and most-cited publication of our aligned electrospun scaffolds for oligodendrocyte culture and myelination:
Bechler, M. E., Byrne, L. & ffrench-Constant (2015). CNS myelin sheath
lengths are an intrinsic property of oligodendrocytes. Current Biology, 25(18), 2411-2416.
Cited >140 times.
RECENT PUBLICATIONS FOR MYELINATION ON MIMETIX SCAFFOLDS
Allimuthu, D., Hubler, Z., Najm, F. J., Tang, H., Bederman, I., Seibel, W., ... & Adams, D. J. (2019). Diverse Chemical Scaffolds Enhance Oligodendrocyte Formation
by Inhibiting CYP51, TM7SF2, or EBP. Cell chemical biology , 26(4), 593-599. Citing
AMS.TECL-006: 12-well Mimetix aligned cell crown inserts.
Bechler, M. E. (2019). A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation. In Oligodendrocytes (pp. 97-110). Humana Press, New York, NY.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
Dillenburg, A., Ireland, G., Holloway, R. K., Davies, C. L., Evans, F. L., Swire, M., Bechler, M. E. ... & Miron, V. E. (2018). Activin receptors regulate the
oligodendrocyte lineage in health and disease. Acta neuropathologica, 135(6), 887-906.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
Elitt, Matthew S., et al. "Chemical Screening Identifies Enhancers of Mutant Oligodendrocyte Survival and Unmasks a Distinct Pathological Phase in
Pelizaeus-Merzbacher Disease." Stem cell reports 11.3 (2018): 711-726.
Citing AMS.TECL-015
: Mimetix aligned 384-well plates (with rhodamine).
Fu, M. M., McAlear, T. S., Nguyen, H., Oses-Prieto, J. A., Valenzuela, A., Shi, R. D., ... Bechstedt, S., & Barres, B. A. (2019). The Golgi Outpost Protein
TPPP Nucleates Microtubules and Is Critical for Myelination. Cell (2019) 179 (1): 132-146.e14.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
See also commentary on this paper
Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M. S., Madhavan, M., Allan, K. C., ... & Nevin, Z. S. (2018). Accumulation of 8, 9-unsaturated sterols
drives oligodendrocyte formation and remyelination. Nature, 560(7718), 372.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
Li, L., Tian, E., Chen, X., Chao, J., Klein, J., Qu, Q., ... & Shi, Y. (2018). GFAP mutations in astrocytes impair oligodendrocyte progenitor proliferation
and myelination in an hiPSC model of Alexander disease. Cell stem cell, 23(2), 239-251.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
Malheiro, A. R., Correia, B., Ferreira da Silva, T., Bessa‐Neto, D., Van Veldhoven, P. P., & Brites, P. (2019). Leukodystrophy caused by plasmalogen
deficiency rescued by glyceryl 1‐myristyl ether treatment. Brain Pathology.
Citing AMS.TECL-003
: 12 well format randomly oriented Mimetix scaffolds.
Tripathi, A., Volsko, C., Garcia, J., Agirre, E., Allan, K., Tesar, P., ... & Dutta, R. (2019). Oligodendrocyte Intrinsic miR-27a Controls Myelination and
Remyelination. Cell-Reports-D-19-02332.
Citing AMS.TECL-006
: 12-well Mimetix aligned cell crown inserts.
Xu, Y. K. T., Chitsaz, D., Brown, R. A., Cui, Q. L., Dabarno, M. A., Antel, J. P., & Kennedy, T. E. (2019). Deep learning for high-throughput
quantification of oligodendrocyte ensheathment at single-cell resolution. Communications Biology, 2(1), 116.
Citing AMS.TECL-005
: 96-well plates with Mimetix aligned fibres.
* AMSBIO is the global source for Mimetix®. Mimetix® is a registered trade mark of and
manufactured by The Electrospinning Company Ltd.