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[UK] +44 (0) 1235 828200 ~ [CH] +41 (0) 91 604 55 22 ~ [DE] +49 (0) 69 77 90 99 ~ [US] +1 800 987 0985


ExpressArt® mRNA AMPLIFICATION AND LABELLING SERVICE

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THE SERVICE PROVIDED BY AMSBIO
Service Outline

  • Initial quality control data and evaluation: photometer and Bioanalyzer.
  • Amplified RNA: quality control with photometer and Bioanalyzer.
  • Labelled RNA (if your order included labelling): quality control with spectrophotometer and Bioanalyzer.
  • Amplified (and if your order included labeling: labelled) RNA samples or Amplified, labelled and fragmented SENSE DNA samples for ST Arrays.




  • Amplification and Labelling

    An internal RNA control using the same master mixes are included. If our internal control does not perform normally, the procedure will be repeated without extra charge. However, if our internal control performs normally, while your samples show insufficient results (yield, incorporation rate, quality), we are not liable for these failures. When performing microarray experiments the RNA quality is important. The performance of the labelling reactions as well as the microarray results will highly reflect the quality of the initial total RNA samples.

    Standard Sample Processing

    RNA samples must meet our specifications (quality, quantity, and concentration):
  • DNase I treated.
  • OD 260/230 > 2.0; OD 260/280 > 1.8.
  • Concentration adjusted > 100 ng/μl or as suspension in ethanol. Please contact us and we will provide a special procedure and reagents for ethanol precipitation.
  • Optional: Gel image or Agilent Bioanalyzer (or BioRad Experion) RNA profile .




  • Labelling Options

  • Biotin-labelled antisense RNA.
  • AminoAllyl-labelled antisense RNA.
  • Cy-dye labelled RNA.
  • Optional fragmented & Biotin-labelled SENSE DNA for ST Arrays (Gene Arrays and Exon Arrays)




  • Specialised sample processing

  • With Expressart® RNA technology very small RNA samples (much less than 100 ng total RNA) can be processed.
  • Degraded RNA samples, including FFPE tissue samples, can be processed with our TR mRNA amplification kits.



  • Initial RNA Quality Control

    An initial control of your RNA upon arrival will be taken using the Agilent Bioanalyzer and the Nanodrop spectrophotometer. You will be informed of the results of the initial quality control.
    Please note that we are not able to check for contaminations with genomic DNA, salts, phenol, or ethanol. The presence of these undetected contaminations can highly affect the labeling reaction.

    If sample processing is terminated at this step, a charge fee will be applied per sample, for sample analysis and data evaluation.
    AMS Biotechnology (Europe) Limited, 184 Park Drive, Milton Park, Abingdon OX14 4SE, UK.
    Telephone: (UK) +44 1235 828 200 (CH) +41 91 604 55 22 (DE) +49 69 77 90 99, VAT No. GB 502 2372 95
    Usage of this web site is governed by our privacy policy, our web site Terms and Conditions,
    our use of the web site Terms and Conditions and our Terms of Sale
    Reg. in England and Wales no. 2117791. ISO 9001 registered firm. ©2013