amsbio offers a range of Directed in vivo Angiogenesis Assay (DIVAA) kits that address the
short-comings of the plug assay by controlling the site for angiogenesis, increasing reproducibility, conserving test
compounds, and supplying more statistical data for quantitative analysis. This range of kits is designed to test for
activation, inhibition and tube formation providing the first complete in vivo
system for the study of angiogenesis that provides quantitative and reproducible
Cultrex® DIVAA Starter Kit
With the onset of angiogenesis, cellular vascularization proceeds to invade the angioreactor, and as early as nine days post-implantation,
there are enough cells to determine an effective dose response to angiogenic modulating factors. The sleek design of the patented angioreactor optimizes sensitivity
and allows for up to four units per mouse, giving a more controlled experiment and decreasing the number of mice required for a study. The DIVAA Starter Kit
was developed with new users in mind and is ideal for optimizing assay conditions.
Cultrex® DIVAA Activation Assay Kit
The DIVAA Activation Kit was designed for assessing angiogenesis activation. The kit contains 48 angioreactors and enough growth factor for eight positive controls. DIVAA has been employed in evaluating the enhancement of angiogenesis associated with adrenomedullin and CD97.
Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon to hold 48 sterile silicone angioreactors in the laminar flow hood while filling and preparing for implantation.
Cultrex® DIVAA Inhibition Kit
The DIVAA Inhibition Kit was designed for assessing angiogenesis
inhibition. The kit contains 48 angioreactors and enough growth factor to induce
angiogenesis in all 48 samples. DIVAA has been utilized in demonstrating the
inhibition of angiogenesis in matrix metalloprotease (MMP)-2-deficient mice and
by TIMP-2. Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon to hold 48 sterile
silicone angioreactors in the laminar flow hood while filling and preparing for implantation. This kit includes pathogen Free BME which has
been tested and cleared of a Battery of 31 pathogens and viruses including LDEV.
Cultrex® in vitro Angiogenesis Assay Tube Formation Kit
Cultrex® in vitro Angiogenesis Assay Kit allows for the detection of inducers and inhibitors of endothelial cell tube formation.
Cultrex® Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS)
tumor, which gels at room temperature to form a reconstituted protein matrix comprised mainly of laminin, collagen IV, entactin, and heparin sulfate proteoglycan.
Sulforaphane [1- isothiocyanato-(4R)-methylsulfinyl)-butane], found in broccoli and other cruciferous vegetables, is a naturally occurring cancer chemopreventive agent,
and is provided as a control for inhibition of in vitro endothelial cell tube formation on Cultrex RGF BME. Calcein AM is provided for rapid and accurate measurement of
cell viability and/or cytotoxicity, and kinetic analysis of tube formation.
The Cultrex® in vitro Angiogenesis Assay Kit provides a cost-effective method for investigation of prospective
angiogenesis inhibitors in a 96 well plate format.
Cultrex® High Protein Concentration Basement Membrane Extract (HC20+) PathClear
Cultrex® PathClear®HC20+ was developed for use in in vivo applications where higher protein
concentrations facilitate faster gelling times, increased gel strength, and elevated levels of tumor augmentation.
Cultrex® PathClear® HC20+ has been tested by PCR and is clear of 31 pathogens and viruses, including LDEV, making it
ideal for in vivo work. Cultrex® PathClear® HC20+ has the advantage of lot-to-lot consistency and controlled protein
concentrations to support in vivo angiogenesis assays and tumorigenicity assays.
Recombinant Animal Free Extracellular Matrix 3D Hydrogels
Tools for 3D Cell Culture
Cultrex® Basement Membrane Extracts (BME)
2D Animal free, chemically defined extracellular matrices
Detachin Cell Detachment Solution