amsbio offers a range of Directed in vivo Angiogenesis Assay (DIVAA™) kits that address the short-comings of the plug assay by controlling the site for angiogenesis, increasing reproducibility, conserving test compounds, and supplying more statistical data for quantitative analysis. This range of kits is designed to test for activation, inhibition and tube formation providing the first complete in vivo system for the study of angiogenesis that provides quantitative and reproducible results.

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Cultrex^® DIVAA™ Starter Kit

With the onset of angiogenesis, cellular vascularization proceeds to invade the angioreactor, and as early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic modulating factors. The sleek design of the patented angioreactor optimizes sensitivity and allows for up to four units per mouse, giving a more controlled experiment and decreasing the number of mice required for a study. The DIVAA™ Starter Kit was developed with new users in mind and is ideal for optimizing assay conditions.

Cultrex^® DIVAA™ Activation Assay Kit

The DIVAA™ Activation Kit was designed for assessing angiogenesis activation. The kit contains 48 angioreactors and enough growth factor for eight positive controls. DIVAA™ has been employed in evaluating the enhancement of angiogenesis associated with adrenomedullin and CD97. Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon to hold 48 sterile silicone angioreactors in the laminar flow hood while filling and preparing for implantation.

Cultrex^® DIVAA™ Inhibition Kit

The DIVAA™ Inhibition Kit was designed for assessing angiogenesis inhibition. The kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all 48 samples. DIVAA™ has been utilized in demonstrating the inhibition of angiogenesis in matrix metalloprotease (MMP)-2-deficient mice and by TIMP-2. Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon to hold 48 sterile silicone angioreactors in the laminar flow hood while filling and preparing for implantation. This kit includes pathogen Free BME which has been tested and cleared of a Battery of 31 pathogens and viruses including LDEV.

Cultrex^® in vitro Angiogenesis Assay Tube Formation Kit

Cultrex^® in vitro Angiogenesis Assay Kit allows for the detection of inducers and inhibitors of endothelial cell tube formation. Cultrex^® Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor, which gels at room temperature to form a reconstituted protein matrix comprised mainly of laminin, collagen IV, entactin, and heparin sulfate proteoglycan. Sulforaphane [1- isothiocyanato-(4R)-methylsulfinyl)-butane], found in broccoli and other cruciferous vegetables, is a naturally occurring cancer chemopreventive agent, and is provided as a control for inhibition of in vitro endothelial cell tube formation on Cultrex RGF BME. Calcein AM is provided for rapid and accurate measurement of cell viability and/or cytotoxicity, and kinetic analysis of tube formation.

The Cultrex^® in vitro Angiogenesis Assay Kit provides a cost-effective method for investigation of prospective angiogenesis inhibitors in a 96 well plate format.

Cultrex^® High Protein Concentration Basement Membrane Extract (HC20+) PathClear

Cultrex^® PathClear^®HC20+ was developed for use in in vivo applications where higher protein concentrations facilitate faster gelling times, increased gel strength, and elevated levels of tumor augmentation. Cultrex^® PathClear^® HC20+ has been tested by PCR and is clear of 31 pathogens and viruses, including LDEV, making it ideal for in vivo work. Cultrex^® PathClear^® HC20+ has the advantage of lot-to-lot consistency and controlled protein concentrations to support in vivo angiogenesis assays and tumorigenicity assays.

Recombinant Animal Free Extracellular Matrix 3D Hydrogels

Tools for 3D Cell Culture

Cultrex^® Basement Membrane Extracts (BME)

2D Animal free, chemically defined extracellular matrices

Detachin™ Cell Detachment Solution