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amsbio a leading supplier of Mouse Embryonic Fibroblasts (MEFs) and Newborn Human Foreskin Fibroblasts (NuFFs) for mouse and
human pluripotent stem cell culture (ES and iPS). Meticulously derived and
comprehensively tested on mouse and human ES stem cells to ensure robust and consistent performance with every lot.
Benefits:
- Minimise Lot to Lot variation
- Eliminate Contamination
- Save Time
| Description |
Datasheet |
Pack Size |
Order |
| CF-1 (Irradiated) High Density |
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7-8 M cells/vial |
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| CF-1 (Irradiated) Standard Density |
|
4-5 M cells/vial
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| CF-1 (Irradiated) Low Density |
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2 M cells/vial
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| CF-1 (Irradiated) Ultra Low Density |
|
0.5 M cells/vial
|
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| CF-1 (Mitomycin C-Treated) Standard Density |
|
4-5 M cells/vial |
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| CF-1 (Mitomycin C-Treated) Low Density |
|
2 M cells/vial |
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| CF-1 (Mitomycin C-Treated) Ultra Low Density |
|
0.5 M cells/vial |
|
| CF-1 (Untreated) Standard Density |
|
4-5 M cells/vial |
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| CF6Neo (Mitomycin C-Treated, Neomycin Resistant) High Density |
|
7-8 M cells/vial |
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| CF6Neo (Mitomycin C-Treated, Neomycin Resistant) Standard Density |
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4-5 M cells/vial |
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| CF6Neo (Irradiated, Neomycin Resistant) Standard Density |
|
4-5 M cells/vial |
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| CF6Neo (Untreated, Neomycin Resistant) Standard Density |
|
4-5 M cells/vial |
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| DR4 (Irradiated, Neomycin, Hygromycin, Puromycin, 6-Thioguanine Resistant) Standard Density |
|
4-5 M cells/vial |
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| DR4 (Irradiated, Neomycin, Hygromycin, Puromycin, 6-Thioguanine Resistant) Low Density |
|
2 M cells/vial |
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| C57BL/6 (Mitomycin C-Treated) Standard Density |
|
4-5 M cells/vial |
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| C57BL/6 (Irradiated) Standard Density |
|
4-5 M cells/vial |
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| C57BL/6 (Untreated) Standard Density |
|
4-5 M cells/vial |
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Every Lot of feeder cells is tested to support embryonic and IPS cells over several passages.
Quality Control:
- 1. PCR‐based assay for 22 mouse pathogens with sensitivity equal to or greater than traditional MAP Testing.
- 2. Direct inoculation and culture of blood agar plates, thioglycolate broth, tryptocase soy broth, and sabaoraud dextrose agar to check for bacterial and fungal contamination.
- 3. PCR‐based assay sensitive to the detection of species typically found as contaminants of cell culture.
- 4. Sparse population of mouse ES cells is plated and observed for overall plating efficiency, colony morphology and immunocytochemistry.
- 5. Qualification based on the morphology, growth, and immunocytochemisty of human ESC cultured for multiple passages.
Latest Publications:
Trilck M et al. (2014) Generation and Neuronal Differentiation of Patient-Specific Induced Pluripotent Stem Cells Derived from Niemann-Pick Type C1 Fibroblasts
Methods Mol Biol.
Ilic D et al. (2014) 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.
Stem Cell Reports Vol. 2, 1–15
Wei Q-X (2012)
Efficient introduction of specific TP53 mutations into mouse embryonic
fibroblasts and embryonic stem cells. Nature Protocols 7, 1145–116.
Technical Downloads:
Feeder Cells FAQs
MEF Cell Culture Protocol
Passaging Pluripotent Stem Cells Protocol
Expanding Untreated Fibroblasts
Mitomycin C Treatment of Fibroblasts
MEF Trouble Shooting Guide
Visit our stem cell research tools pages
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