amsbio transfection reagents include
GeneSilencer®
with a unique cationic lipid formulation for efficient transfection of siRNA into mammalian cells.
- High siRNA transfection efficiency
- Functional gene silencing post siRNA delivery
- Compatibility with diverse growth conditions (with and without serum)
- Low cytotoxicity
- Easy-to-use protocols for both adherent and suspension cells
GeneSilencer® siRNA transfection Reagent is a novel cationic lipid
formulation specifically designed for efficient delivery of siRNAs (small
interfering RNAs) into a wide variety of cell types. siRNAs are short,
gene-specific double-stranded RNAs that can cause gene silencing in mammalian
cells by catalytically cleaving greater than 95% of the target mRNA (1,2,3).
GeneSilencer® Reagent vs. siPORT™ NeoFX™ Reagent
Figure Legend: HEK 293 cells were transfected with 5, 10, 30 or 50 nM of Silencer® FAM Labeled GAPDH siRNA with
either GeneSilencer® or siPORT NeoFX™ (Ambion) siRNA transfection reagents according to the manufacturer's recommended protocols.
Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor® 555 phalloidin (Invitrogen), which stains cellular
actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells
were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA
is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
Contents:
Hydrated GeneSilencer® lipid (1 vial x 750 ul for T500750 or 5 vials x 750 ul for T505750)
siRNA Diluent (1 tube x 4 ml for T500750 / 5 tubes x 4 ml for T505750)
Storage:
The GeneSilencer® siRNA transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4oC upon receipt. If stored properly, all components are stable for 6 months.
References:
1. Elbashir, S.M. et al. (2001) Nature 411: 494-498.2
2. Caplen, N.J. et al. (2001) proc Natl Acad Sci USA 98: 9742-9747.
3. Sharp, p.A. (2001) Genes and Development 15: 485-490.