Product Search:
Customer Service Team direct on (UK) +44(0)1235 828 200 : (CH) +41(0)91-604 55 22 : (DE) +49(0)69-77 90 99

siRNA Transfection Reagent GeneSilencer
amsbio.com - siRNA Transfection Reagent GeneSilencer
amsbio transfection reagents include GeneSilencer® for the efficient transfection of siRNA into mammalian cells.
  • High siRNA transfection efficiency
  • Functional gene silencing post siRNA delivery
  • Compatibility with diverse growth conditions (with and without serum)
  • Low cytotoxicity
  • Easy-to-use protocols for both adherent and suspension cells

ORDER NOW

GeneSilencer® siRNA transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types.

  • GeneSilencer Protocol
    		•
  • 10 Tips For Gene Silencing
  • Application Note
    		•
  • Primary Cell Citations
  • GeneSilencer FAQs
    		•
  • All GeneSilencer Citations
    •  
    'In contrast [to the Mouse Nucleofector kit from Amaxa Biosystems and transfection reagents from Santa Cruz Biotechnology], the most effective method was the lipid-based method using the siRNA transfection reagent GeneSilencer. This protocol resulted in up to 92% FITC-siRNA positive cells after 4 h which declined to 62% and 59% 24 and 48 h post-transfection, respectively. The transfected BM-DC remained CD11c positive, expressed high MHC class II and intermediate CD40 and were functional as ApC.'
     
    Pedersen, CD, Fang, JJ, and Pedersen, AE, (2009) A comparative study of transfection methods for RNA interference in bone marrow-derived murine dendritic cells. Scand J Immunol 70(5): 447.
     
    Description:

    GeneSilencer® siRNA transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types. siRNAs are short, gene-specific double-stranded RNAs that can cause gene silencing in mammalian cells by catalytically cleaving greater than 95% of the target mRNA (1,2,3).

    Contents:

  • Hydrated GeneSilencer® lipid (1 vial x 750 ul for T500750 or 5 vials x 750 ul for T505750)
  • siRNA Diluent (1 tube x 4 ml for T500750 / 5 tubes x 4 ml for T505750)

    • Storage:
    The GeneSilencer® siRNA transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at 4oC upon receipt. If stored properly, all components are stable for 6 months.

    References
    1. Elbashir, S.M. et al. (2001) Nature 411: 494-498.
    2. Caplen, N.J. et al. (2001) proc Natl Acad Sci USA 98: 9742-9747.
    3. Sharp, p.A. (2001) Genes and Development 15: 485-490.

    		            

    293 cells transiently transfected with 1 ug of gWiz/GFp. When they are co-transfected with 500 ng of GFp siRNA, GFp expression was efficiently suppressed. (GFp: green, left panels. DApI: blue, right panels)

    Also Available

     
  • Dicer siRNA Generation Kit
    		•
  • Recombinant Human Turbo Dicer Kit
  • GenePORTER® 3000 transfection Reagent
    		•
  • Detachin™ Cell Detachment Solution
  • MycoGONE™ Mycoplasma Antibiotics Cocktail
    		•
  • FACSmax™ Cell Dissociation Solution

    •  
    Feature Areas