amsbio supplies CometAssay® provides reagents and our exclusive CometSlideTM for the rapid analysis of DNA fragmentation associated with DNA damage. The comet assay or single cell gel electrophoresis assay is based on the alkaline lysis of labile DNA at sites of damage. The unwound, relaxed DNA is able to migrate out of the cell during electrophoresis and can be visualized using SYBR® Green I nucleic acid gel stain. Cells that have accumulated DNA damage appear as fluorescent comets with tails of DNA fragmentation or unwinding, whereas, normal undamaged DNA does not migrate far from the origin. The CometAssay® is provided with our exclusive CometSlidesTM which greatly simplify the assay. Each slide provides two sample surfaces specially treated to promote agarose adherence, and a hydrophobic barrier to allow treatment with DNA repair enzymes. Simply add your cells to the low melting point Comet LMAgarose, and pipet onto the slide.
Ordering Infomation
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Features:
CometSlidesTM allow direct application of Comet LMAgarose without base layers or pretreatment.
Hydrophobic barrier allows easy application of cells directly to slide, and treatment with any of several DNA repair enzymes.
Easy to use kit format. |
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Applications
Detect and quantitate DNA damage
Identify apoptotic cells quickly and easily
Follow DNA repair
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| Part # |
Component |
Size |
| 4250-050-01 |
CometAssay Lysis Solution |
2 x 500 ml |
| 4250-050-02 |
CometAssay LMAgarose |
15 ml |
| 4250-050-03 |
CometSlide (2 well slide) |
25 each |
| 4250-050-04 |
200 mM EDTA; pH 10 |
12.5 ml |
| 4250-050-05 |
SYBR Green |
5 ul |