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FACSmax Cell Dissociation Solution
amsbio.com - FACSmax Cell Dissociation Solution
amsbio supplies the new FACSmax™ Cell Dissociation Solution the gentle and highly effective cell dissociation solution.
  • Dissociates clumped cells in minutes
  • Results in homogeneous single cell suspension
  • Gentle cell disaggregation for maximum cell viability
  • Yields accurate, reproducible cell counts
  • Saves time - No need for extra PBS washing steps
  • Ready to use solution
 
FACSmax Datasheet                     Cell_Dissociation_Reagent Application Note
 
 
This proprietary formulation of proteolytic, collagenolytic and DNAse enzymes is highly effective in creating single cell suspensions from clumped cell cultures for accurate cell counting, flow cytometry, viral transfection assays, cell sorting, and bioreactor scale-up.
 
 
Untreated MCF-7 Cells FACSmax™ treated MCF-7 Cells
Cell Dissociation Solution FACSmax

MCF-7 cells were harvested at 90% confluency from a T150 culture flask using a cell scraper, centrifuged and resuspended in 10 mls of complete medium. A. 0.10 mls of suspended cells were transfered to an eppie tube, to which 0.10 mls of PBS was added. The cells were vortexed and incubated @ room temperature for 5 mintues then mounted on a glass slide and photographed. MCF-7 cells are adherent and grow in clumps. They are very difficult to dissociate. B. 0.10 mls of suspended cells were transfered to an eppie tube, to which 0.10 mls of cold FACSmax™ cell dissociation solution was added. The cells were vortexed and incubated @ room temperature for 5 mintues then mounted on a glass slide and photographed. After treatment with FACSmax™ a single cell suspension makes for easy and accurate cell counting.

 
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