FACSmax Cell Dissociation Solution the gentle and highly effective cell dissociation solution.
- Dissociates clumped cells in minutes
- Results in homogeneous single cell suspension
- Gentle cell disaggregation for maximum cell viability
- Yields accurate, reproducible cell counts
- Saves time - No need for extra PBS washing steps
- Ready to use solution
Martha Manoli & Wolfgang Driever (2012)
Fluorescence-Activated Cell Sorting (FACS) of Fluorescently Tagged Cells from Zebrafish Larvae for RNA Isolation. Cold Spring Harb Protoc.
This proprietary formulation of proteolytic, collagenolytic and DNAse enzymes is highly effective in creating single
cell suspensions from clumped cell cultures for accurate cell counting, flow cytometry, viral transfection assays,
cell sorting, and bioreactor scale-up.
|Untreated MCF-7 Cells
||FACSmax treated MCF-7 Cells|
Figure Legend: MCF-7 cells were harvested at 90% confluency from a T150 culture flask using a cell scraper, centrifuged and resuspended
in 10 mls of complete medium. A. 0.10 mls of suspended cells were transfered to an eppie tube, to which 0.10 mls of PBS was added.
The cells were vortexed and incubated @ room temperature for 5 mintues then mounted on a glass slide and photographed.
MCF-7 cells are adherent and grow in clumps. They are very difficult to dissociate. B. 0.10 mls of suspended cells were transfered
to an eppie tube, to which 0.10 mls of cold FACSmax cell dissociation solution was added. The cells were vortexed and incubated
@ room temperature for 5 mintues then mounted on a glass slide and photographed. After treatment with FACSmax a single cell
suspension makes for easy and accurate cell counting.