PHASE 1 : IMMUNIZATION AND ELISA EVALUTATION
Immunization and boosts of 5 Balb/c mice with standard immunization protocol.
ELISA evaluation of titer prior to selection for fusion.
Approximately 3-4 mg protein immunogen or 3 mg conjugated peptide immunogen and 1 mg free peptide are required.
Estimated time: 8~10 weeks.
PHASE 2 : HYBRIDOMA FUSION AND SCREENING OF SPECIFIC CLONES
Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from the mouse with the best titer and myeloma cells.
Supernatants from the growing hybridoma wells will be screened by ELISA. Supernatant from 10 different ELISA positive clones will be sent to customer's lab for evaluation.
Estimated time: 4~5 weeks.
PHASE 3 : SUBCLONING
Two of these ten clones selected by the customer will be subcloned by limiting dilution, isotyped, and the cell line will be shipped to the customer.
Estimated time: 6~8 weeks.
Note: Production and purification services avaiable. Please enquire.