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MAMMALIAN PROTEIN EXPRESSION

QUALITY YOU CAN TRUST
  • High level expression system
  • Competitive price
  • Human cell line origin with full glycosylation
  • Scale up to 500 liters
  • Tag removel as requested

SERVICE

DESCRIPTION

baculovirus-protein-draw
PHASE 1 :
  • Cloning of your interest gene into vector with His-tag or IgG-Fc tag.
  • Sequencing confirmation of insert.
  • Small scale (5ml) transfection to test the expression level and optimization.
  • Estimated time: 3-4weeks
PHASE 2 :
  • Large scale transfection (500ml) of recombinant vector into HEK293 and protein expression, evaluation, and optimization.
  • Protein purification from 500ml of HEK293 culture using affinity column of Ni-NTA or Protein A (No tag cleavage service provided).
  • The yield ranks from 3 to 25mg per 500ml with an average of 5mg.
  • Estimated time: 2-3 weeks
DELIVERABLE FROM PHASE 2 :

Protein and report including sequence confirmation, Western blot and SDS-PAGE result, design form.