|
|
SERVICE |
DESCRIPTION |
TIME |
| 1
|
Peptide Synthesis |
-
Custom FMOC Peptide synthesis up to 18 amino acids (20-30 mg). Peptide with
more than 18 amino acids will be charged pro rata. We can provide assistance on
designing peptide with respect to protein homology, antigenicity,
hydrophilicity, and synthetic suitability
-
HPLC purification if peptide purity is < 90%
|
1.5 weeks |
| 2
|
Conjugation |
-
Conjugation of 4 mg peptide (using Cysteine) to KLH
-
Conjugation to IKERSATM (a unique 28 kDa recombinant protein
from Immunokontact, conjugation using Cysteine residue at the N-terminal of
peptide) for western blotting analysis and other functional studies.
|
0.5 weeks |
| 3
|
Immunization
and anti-sera
production |
-
Conjugated peptides or recombinant proteins are used to immunize rabbits.
-
Ten-week protocol to produce antibodies in 2 rabbits.
-
Pre-immune and three immune bleeds (100-120 ml antiserum).
|
7-10 weeks |
| 4
|
Test |
-
ELISA test until the titre reaches 1:4,000
-
Western Blot analysis when ELISA reaches target titre
|
1 day |
| 5
|
Purification |
-
Protein G purification
-
Antibodies are eluted using both pH11 and pH2.5 buffers and neutralized
immediately followed by dialysis in PBS
|
1 week |
| 6
|
Total |
|
10-13
weeks |
|
|
SERVICE |
DESCRIPTION |
TIME |
| 3
|
Immunization
and anti-sera
production |
-
Add for each additional rabbit
-
Extend protocol for each additional week
|
|
| 4
|
Test |
-
Immunohistochemistry on tissues or cultured cells
-
Immunoprecipitation on tissue or cell lysate
|
1 week
1 week |
| 5
|
Purification |
-
Peptide affinity purification
|
1 week |
