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AMSBIO CUSTOM SERVICE : STABLE CELL LINE GENERATION REQUEST FORM

SERVICE REQUIREMENTS
1. Host cell line:
Name:
Can you provide the host cell line?
Company name:
Cat#:
Please note that the construction of a stable cell line in our 293HEK Cells (Human Embryonic Kidney) might be a cheaper option.
For generation of a stable cell line over-expressing a specific cDNA, please answer questions 2 to 6.
2. Gene accession number (mRNA NCBI reference):
Can you provide the DNA gene template (along with its coding sequence) for sub-cloning?
3. Please select the promoter you would prefer for the gene:
Please, specify:
4. Do you need a tag (6His-tag, HA, Myc, V5, GFP, RFP, CFP...)?
Please, specify the tag you prefer:
Please, specify the tag you prefer:
5. Please select the marker you prefer:
6. Expression validation in Western Blot:
Details :
For generation of a KNOCKDOWN stable cell line, please answer questions 7 to 9 :
7. Gene accession number (mRNA NCBI reference):
Can you provide the knockdown sequence?
8. Constitutive or inducible expression of the shRNA?
9. Please select the marker you prefer:
Additional stable cell line requirements or comments (Mutagenesis, negative control cell line, bicistronic expression etc.) :
CONTACT DETAILS
Date/Time of Request:19/05/2013 08:32:45
Name(*): 
Telephone Number(*): 
FAX Number:
Email Address(*): 
Organisation(*): 
Department:
Company:
Your Reference Number:
Address(*): 
Post Code(*): 
Country:
Purchase Time Frame(*): 
For further information, please email info@amsbio.com.
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Your Email Address: Colleague's Email Address:
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