Over-expression lentivirus construction:
You choose the promoter, a tag, a selection marker and a fluorescent marker (see vectors map scheme).
Why use AMSBIO lentivirus services :
Safe-to-use (self-inactivating) lentiviral particles can deliver your gene into a wide range of cell lines including non-dividing, primary or stem cells.
Engineered in-house lentiviral vector for highly efficient gene integration into cell genome.
Our lentivectors have the strongest promoter for the highest protein expression in mammalian cells.
You can choose to have an inducible* or constitutive expression.High titer of lentivirus that is monitored by a fluorescent protein (not fused with your target).
Our experts with years of experience in lentiviral cloning and expression.
Fast turnaround time.
The best price and the best quality in its class.
Either you provide target templates or we synthesize or obtain it from a cDNA collection.
We construct the lentiviral clones and generate 0.5 ml (or more if requested) of ready-to-use expression lentivirus.
Some of AMSBIO's other services :
High titer ready-to-use shRNA Lentivirus for your targets (around 107 TU/ml).
Generation of stable cell lines in almost any mammalian cell line.
Protein expression in mammalian suspension cells using lentiviral transduction.
Affinity purification (His-tag) of recombinant proteins expressed in mammalian cells.
* Additional information on our inducible suCMV promoter :
Without further intervention, our inducible lentiviral particles drive regular constitutive expression.
To take advantage of the promoter’s inducible properties, a repressor protein TetR must be present. The expression of TetR can be achieved by using our
pre-made Tet-repressor lentiviral particles or by using our
stable Tet-repressor cell line.
Initially, the expression of the target protein is repressed by TetR, but addition of tetracycline induces expression in a dose dependant manner.
Please note that although our lentiviral vectors contain all necessary bio-safety features, work with lentiviral particles should be carried out under
Biological Safety Level 2 (BL2)
Please conduct a thorough risk assessment for your project and contact your health and safety facilities for local guidelines and regulations.