MAPTrix - Recombinant Animal Free Extracellular Matrix
MAPTrix extracellular matrix
MAPTrix™ extracellular matrix (ECM) based coatings or surface modification is simple,
convenient and highly reproducible. You can readily engineer a synthetic ECM surface
that binds to adhesion receptors such as integrins and promote cell adhesion and spreading.
AMSBIO supplies Mussel Adhesive Protein based matrix (MAPTrixTM)
recombinant extracellular matrix (ECM) that act as biometic mimics for traditional basement membrane extracts.
MAPTrixTM replaces traditional ECM with genetically incorporated bioactive peptides (recognition peptides)
that provide an environment for the maintenance of cells under serum and feeder-free conditions.
Benefits of using MAPTrix extracellular matrix from AMSBIO
- Biochemically-defined, animal-free cell culture surfaces designed to enhance cell performance.
- Adhesion properties of the mussel adhesive protein makes the coating reproducible & reliable.
- MAPTrix™ produces a uniform ECM surface that provides a highly controlled 2D extracellular microenvironment for cell cultures and related applications.
- A wide array of MAPTrix™ ECM products are currently available (115 ECM lines).
- Low cost compared to traditional ECM.
- Eliminates risk of animal or viral infectious agents in cell cultures.
- Helps comply with FDA recommendations for animal-free component.
- Ready to use and compatible with standard coating protocols.
- Improved cell morphology and cell proliferation.
The MAPTrixTM ECM line of products are provided with a guaranteed purity
of >90% (SDS-PAGE) with endotoxin levels below 20 EU/mL (LAL assay). Each
product is tested for the presence of bacteria, fungi and mycoplasma with biological activity of each product determined in
a cell culture assay under serum-free conditions.
MAPTrix ECM Array Technology
A promising tool for the in vitro exploration of extracellular microenvironment (ECM) factors that regulate cell functions
is the combinatorial library approach. With the use of combinatorial libraries, one can explore the effect of numerous know and
unknown factors, alone or in complex combinations in analogous to those encountered in natural extracellular microenvironments.
Our MAPTrix™ ECM Library is the first commercially available combinatorial synthetic ECM library. It is comprised of hundreds
of ECM derived peptide motifs which primarily bind to specific integrins. When combined with high throughput technology, our
MAPTrix™ Array based cell culture systems allows one to investigate and/or optimize ECM cues and cellular signaling events which
control cellular functions. The information obtained from the cell culture systems can be translated and applied to creating or
engineering biomimetic or synthetic microenvironments in order to regulate cell behavior for biomedical applications.
MAPTrix™ Array offers an extensive line of ECM-derived ligands for high throughput cell adhesion assays to identify a cellular
adhesion profile against up to 300 cell surface receptor binding peptide motifs.
Below is an example of the data obtained from an ECM array.
Primary HUVEC cells were incubated on MAPTrix™ ECM arrays for 48 hours in serum free conditions. Cell counts were normalized against
average cell counts on Non-coating (BD Falcon). Each bar represents the mean value of three wells.