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shRNA Plasmids (162442)
amsbio offers HuSH-29 Genome-wide shRNA for Human, Mouse and Rat Genes.
HuSH-29 are pre-designed shRNA with genome wide coverage (human, mouse and rat) that delivers guaranteed knockdown. siRNAs can be
synthesized and transfected into mammalian cells, resulting in effective suppression of
gene expression. However such suppression is transient. By contrast, short hairpin
RNAs (shRNA) can suppress gene expression over a prolonged period by continually
expressing an RNA duplex (Brummelkamp 2002; Paddison 2002).
Download Manual
Features and Benefits
- Three retroviral vector options:
- pRS: basic retroviral vector without fluorescence
- pRS with GFP to monitor transfection
- pRS with RFP, ideal for double knockdown
- Guaranteed successful gene knockdown (≥70%)
- Versatile applications: transient or stable transfection or retroviral infection
- Low cost kits (4 gene-specific shRNA and two negative controls)
- 5 μg transfection-ready DNA using PowerPrep® HP Kits
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