Proteins - Lentiviral Particles (325)

Seeing is believing!

Expression ready lentiviral particles are the next generation gene delivery tool. These premade particles contain a specific gene that is ready to be delivered into a wide range of cell types including primary cells and non-dividing cells. Co-expressed RFP (non-fusion) by 2A peptide processing (SureTiter^TM) or RSV promoter (Inducible system) enables real-time monitoring of protein expression.

Applications for premade lentiviral particles:

• Deliver genes for expression or knock-down in hard-to-transfect cell types (such as primary cells, stem cells and drug-arrested cells).
• Create stable cell lines for long-term high level expression easily and cost effectively.
• High constitutive expression or Tet inducible expression in the presence of TetR (a repressor protein).
• Transduction protocols for adhesive and suspension cells.
• Can also be used in vivo to generate transgenic animals.
• Organelle targeting for sub-cellular localization analysis (in development)

Features of premade lentiviral particles:

• Easy and Ready-to-use: directly added into dividing and non-dividing cells.
• No need any lipids or transfection reagents.
• Deliver genes in highly a reproducible and controllable method.
• Safe to use: Self-inactivation to prevent viron replication.
• High true titer: 1 x 10⁷ IFU/ml or higher.
• Real-time monitoring of expression after 48-72 hours via co-expressed RFP fluorescent signal.
• Native and HIS tagged proteins available

Using our SureTiter^TM lentiviral vectors or Inducible lentiviral vectors we are able to generate robust lentiviral particles. Our titers are validated by true infectious functional units using fluorescent cells rather than by p24 ELISA or qPCR (which do not reflect true titers).

Particles are packaged in culture medium with 10% FBS, or in serum-free medium.

FAQ about Ready-to-use lentiviral particles

Product series:

• The complete ready to use Lentivirus Particles list

Specific categories:

• Ready-to-use particles for fluorescent markers:
  • GFP / CFP / RFP / YFP .
• Ready-to-use particles for inducing pluripotent stem cells (iPSC):
  • (Complete set, NANOG, OCT4,SOX2, KLF4, Myc, LIN28).
• Ready-to-use particles for enzymes and markers.
• Ready-to-use particles for human genes.
• Ready-to-use particles for mouse genes.
• Ready-to-use Serum-free lentivirus particles.
• Ready-to-use HIS-tagged expression particles.
• Ready-to-use lentivirus particles for in vivo use.

Do-it-yourself Lentiviral packaging

• Lentiviral cloning kits and packaging plasmids.
• 293t lentiviral / lentivirus packing cells.

References:

• Delenda, C. et al., Lentiviral vectors: optimization of packaging, transduction and gene expression. The J of gene medicine. 6. S125-s138 (2004).
• Zufferey, R., et al., Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol. 72, 9873-80 (1998).
• Tom Dull, et al., A third-generation lentivirus vector with a conditional packaging system. J Virol. 72. 8463-8471 (1998).
• Mukesh Kumar, et al., Large-Scale production of pseudotyped lentiviral vectors using baculovirus GP64. Human gene therapy. 14:67-77 (2003).
• NIH Guidelines for Biosafety Considerations for Research with Lentiviral Vectors.
• CDC guidelines for Lab Biosafety levels
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