AMSBIO supplies a range of high viability frozen primary rat neural cells, offering a convenient source of rat neural cells.
Primary Rat Cortical Neurons
The cerebral cortex plays an important role in memory, higher thinking, perception and the understanding of language. These cryopreserved Primary Rat Corcal Neurons (RCN) are isolated
from early embryonicstage (E18) Sprague Dawley Rats and provided cryopreserved asprimary cells. Each vial of rat corcal neurons contains at least 4 million viable cells, with post thaw viability
consistently in the range of 50-80%
Figure 1. Long-Term Viability - Rat cortical neuronsremain viable during long term culturing.
Above images show cells at 1 week (A), 2 weeks (B) and 3 weeks (C) in culture.
Figure 2. Healthy, Mature Cortical Neurons Post-thaw - Primary rat cortical neurons were isolated from Sprague-Dawley (E18) rats. MAP-2 (green)
staining shows that greater than 90% of the isolated cells are neurons. Dapi staining (blue) denotes cell nuclei.
Figure 3. Evoked Synaptic Release Activity - Cryopreserved and Freshly isolated primary rat neurons were each plated at 80,000 cells per well and grown for 3 weeks.
All the cells in the well were stimulated simultaneously. Above data shows cryopreserved primary rat neurons exhibit evoked synaptic release responses virtually identical to that of fresh isolated rat forebrain neurons.
The fluorescent readout was a measure of pre-synaptic release. Data courtesy of Pascal Laeng (Galenea).
Figure 4. Neuronal Transfection - Rat Cortical Neurons were transfected with GeneIn-CNS at high efficiency, while maintaining maximal cell viability.
Rat Glial Restricted Precursor Cells
Figure 5. Healthy, Undifferentiated GRP's - Rat Glial Restricted Progenitors (rGRP's) isolated from Sprague-Dawley rats, postnatal day 3. The above image shows undifferentiated
rat GRP's characterized by A2B5 (green) staining. Cells nuclei are stained with Dapi (blue).
Figure 6. Healthy, mature Astrocytes - Rat Glial Restricted Progenitors (rGRP's) isolated from Sprague-Dawley rats, postnatal day 3. The above image shows differentiated Astrocytes
characterized by GFAP (green) staining. Cells nuclei are stained with Dapi (blue).
Figure 7. Healthy, mature Oligodendrocytes - Rat Glial Restricted Progenitors (rGRP's) isolated from Sprague-Dawley rats, postnatal day 3. The above image shows differentiated
oligodendrocytes characterized by GalC (green) staining. Cells nuclei are stained with Dapi (blue).
Primary Rat Astrocytes
The cerebral cortex plays a key role in memory, learning, consciousness and the understanding of language. Primary
Rat Astrocytes isolated from early embryonic stage (E19) Sprague-Dawley Rats are cryopreserved at primary passage. Derived from cortical tissue, Primary Rat Astrocytes are used to support Primary Neurons for electrophysiology and in the study of
glial scar formation, neurotransmitter synthesis and recycling. Post thaw viability is in the range of 70-80%. Each vial contains approximately 1 million viable cells.
Figure 8. Healthy, Mature Astrocytes - Primary Rat Astrocytes isolated from E19 Sprague-Dawley Rats. The above image shows differentiated Primary Rat Astrocytes characterized by GFAP
(red) staining. Cells nuclei are stained with Dapi (blue).
Primary Rat Neural Stem Cells
Primary neural stem cells isolated from the cortical neuroepithelium of the fetal rat (embryonic day 14), P0. They are reproducible and multipotent. After plating, the cells are ready for expansion or differentiation into neurons, astrocytes
and oligodendrocytes. They can be transfected, transplanted or differentiated into electrically active neurons. We take care of everything from dissection and isolation to cryopreservation. Plus, we perform extensive quality control and
performance testing on every batch.Undirected differentiation to neurons, astrocytes and oligodendrocytes can be achieved by the withdrawal of the mitogen basic fibroblast growth factor (bFGF).
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Figure 9. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, MAP2 (red), and the astrocyte marker, GFAP (green).
The nuclei are stained with DAPI (blue).
Figure 10. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, TuΒ3 (left, green), and the astrocyte marker, GFAP (right, green).
The nuclei in both images are stained with DAPI (blue).