MycoGONE™ Mycoplasma Antibiotics Cocktail
Powerful and effective antibiotics that kill mycoplasma
- Effective mycoplasma removal
- No effect on eukaryotic cell proliferation
- Easy to use
amsbio now provides a proven disinfection solution for cleaning up mycoplasma-contaminated cells with MycoGONE™. This powerful and effective combination of antibiotics kills all known mycoplasma strains and is safe for mammalian cell culture use.
Applications:
- Mycoplasma infected established cell line
- Decontamination of primary material
- Prophylaxis for cell culture
Treatment with MycoGONE™ involves three or more cell passaging cycles. During treatment, check the culture every week for the presence of mycoplasma using any available methods at your disposal, such as ELISA, fluorescence staining, or PCR. For easy and quick detection, we recommend the
MycoScope kit. By using this protocol, you can expect to remove all traces of mycoplasma contamination within 2-3 weeks.
MycoGONE™ can be used safely at the following dilutions:
1:50 (1X final); 1:40 (1.25 X final); 1:30 (1.67X final); 1:25 (2X final).
Protocol:
- Dilute MycoGONE™ 50-fold to a 1X concentration in the appropriate media used for your cell cultures.
- Remove medium from contaminatied culture vessels, wash the cells with PBS, then detach cells with Detachin or Trypsin-EDTA using the suppllier's recommended protocol.
- Count the cells and plate in fresh medium containing MycoGONE™ from Step 1 above.
- Incubate cells for 2-3 days as you would typically.
- Remove the media, wash the cells with PBS, then detach as in Step 2 above.
- Count the cells and plate in fresh medium containing MycoGONE™ prepared in Step 1 above.
- Incubate cells for 2-3 days.
- Repeat Steps 5 through 7 two more times.
- Check for mycoplasma contamination; make sure to use positive and negative controls if available for accurate diagnosis.
- If mycoplasma contamination persists, increase MycoGONE™ concentrations by reducing the dilution factor and repeating the treatment cycle in Steps 2 to 9.