MycoGONE™ Mycoplasma Antibiotics Cocktail
Powerful and effective antibiotics that kill mycoplasma
- Effective mycoplasma removal
- No effect on eukaryotic cell proliferation
- Easy to use
amsbio now provides a proven disinfection solution for cleaning up mycoplasma-contaminated cells with MycoGONE™. This powerful and effective combination of antibiotics kills all known mycoplasma strains and is safe for mammalian cell culture use.
Mycoplasma contamination is a serious threat to cell culture based laboratory research. Studies have found as many as 85% of cell cultures tested show signs of contamination. This is not surprising considering the multiple sources of contamination, such as serum, media, additives, laboratory personnel, and faulty equipment (tissue culture hoods or incubator chambers).Mycoplasma's negative impact tends to go unchecked due to the difficulty in detecting these ubiquitous bacteria. Widespread Mycoplasma contamination in a cell culture laboratory can lead to costly and time-consuming clean-up procedures Thus, it is highly recommended to perform regular screenings, and proven disinfection methods if mycoplasma contamination is found.
ORDERING INFO
Applications
Mycoplasma infected established cell line
Decontamination of primary material
Prophylaxis for cell culture Notes
Treatment with MycoGONE™ involves three or more cell passaging cycles. During treatment, check the culture every week for the presence of mycoplasma using any available methods at your disposal, such as ELISA, fluorescence staining, or PCR. For easy and quick detection, we recommend the
MycoScope kit. By using this protocol, you can expect to remove all traces of mycoplasma contamination within 2-3 weeks.
MycoGONE™ can be used safely at the following dilutions:
1:50 (1X final); 1:40 (1.25 X final); 1:30 (1.67X final); 1:25 (2X final).
PROTOCOL
- Dilute MycoGONE™ 50-fold to a 1X concentration in the appropriate media used for your cell cultures.
- Remove medium from contaminatied culture vessels, wash the cells with PBS, then detach cells with Detachin or Trypsin-EDTA using the suppllier's recommended protocol.
- Count the cells and plate in fresh medium containing MycoGONE™ from Step 1 above.
- Incubate cells for 2-3 days as you would typically.
- Remove the media, wash the cells with PBS, then detach as in Step 2 above.
- Count the cells and plate in fresh medium containing MycoGONE™ prepared in Step 1 above.
- Incubate cells for 2-3 days.
- Repeat Steps 5 through 7 two more times.
- Check for mycoplasma contamination; make sure to use positive and negative controls if available for accurate diagnosis.
- If mycoplasma contamination persists, increase MycoGONE™ concentrations by reducing the dilution factor and repeating the treatment cycle in Steps 2 to 9.