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Mouse Leukemia Inhibitory Factor (mLIF)
PluriQ™ Qualify for use with mouse embryonic stem cells
amsbio now offers recombinant mLIF at exceptional value and high quality. Batch tested in mouse ES cells, mLIF promote long-term maintenance and propagation of undifferentiated mouse embryonic and induced pluripotent stem cells.
Low cost per unit of activity - 5ng = 1000 Units
Qualified with human and mouse pluripotent stem cells over multiple passages
Tested for sterility and mycoplasma
No pyrogenic or pro-inflammatory contaminants
Low endotoxin levels
Customer Assay
Bioactivity was tested by culturing mouse ES cells either on feeders in complete medium or without feeders in a conditioned medium. In both conditions mLIF was added
using concentrations down to 0.1 ng/ml. All concentration conditions resulted in nice looking colonies of pluripotent mouse ES cells. When the ES cells were deprived of mLIF cells spontaneously
differentiated and were able to form embryoid bodies in hanging drops.
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which is involved in a number of reproductive, inflammatory, developmental and regenerative processes.
LIF promotes long-term maintenance of pluripotent stem cells by suppressing spontaneous differentiation. LIF is also involved in a number of other biological processes including neural and hematopoietic cell differentiation,
bone and fat metabolism, and mitogenesis of certain factor dependent cell lines. Each lot is PluriQ™ tested on mouse embryonic stem cells to confirm its functionality in plating efficiency, colony formation, morphology and
maintenance of the undifferentiated state.
Related Areas
Recombinant Animal Free Extracellular Matrix 3D Hydrogels
Cultrex® Basement Membrane Extracts (BME)
2D Animal free, chemically defined extracellular matrices
Visit our stem cell research tools pages.
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