What's in your Monoclonal?
amsbio supplies the Iso-Gold Rapid Mouse-Isotyping Kit the 5 minute lateral flow assay for monoclonal antibody class and subclass determination.
- Determine the isotype of your monoclonal antibody
- assess the purity of your purified monoclonal antibody*
- Verify the purity of your purchased monclonal antibody*
- Reduce runtime
*multiple lines apear and indicate subclass contamination
Iso-Gold kits provide a colour readout in 5 minutes producing a validated
result of mouse monoclonal antibody isotope (IgG1, IgG2a, IgG2b, IgG3, IgA or IgM) and
are now available with kappa and lambda light chains determination.
Comparison with ELISA format isotyping assay 
Isotyping kit comparison
|
EIA Tests |
|
Company |
Format |
# of Tests per Kit |
# of wells needed per antibody |
Test Time |
Specifics |
Sensitivity |
|
amsbio |
Rapid Lateral Flow |
10 |
N/A |
|
+ light chains |
10 ng/ml |
|
AbD serotec |
Latex Lateral Flow (Roche) |
10 |
N/A |
5 - 10 min |
+ light chains |
not listed |
|
BD |
EIA |
80 |
12 |
8 hr |
+ light chains |
not listed |
|
BioRad |
EIA |
100 |
8 |
8 hr |
+ light chains |
not listed |
|
ClonaCell
(EY Labs) |
Flow Through |
20 |
N/A |
1 min - 3 steps |
does not include IgA/ no light chains |
1 ug/ml |
|
Invitrogen (Zymed) |
EIA |
100 |
8 |
8 hr |
+ light chains |
50 ng/ml |
|
Pierce |
EIA |
100 |
8 |
8 hr |
+ light chains |
not listed |
|
Roche |
Latex Lateral Flow |
10 |
N/A |
5 min |
+ light chains |
50 ng/ml |
|
Calbiochem
(reagents to make EIA test) |
EIA |
100 |
6 |
2 days, 10 plates |
Mab isotypes /no light chains |
|
|
Note: All of the EIA Test companies listed only provide materials to make your own kit. |