ImmunoPure™ PBMCs
AMSBIO know that researchers have more important work at hand than struggling with procuring tissue and isolating cells.
We have established a robust tissue procurement network and world class cell isolation technologies in order to simplify research that requires
primary cells.
AMSBIO now introduces the new
range of ImmunoPure™ peripheral blood mononuclear cells (PBMCs). A variety of
immune cells derived from the peripheral blood of normal healthy human adult donors collected at IRB-approved blood banks.
- Proprietary reagents ensure high survival and recovery rates
- Analyzed and characterized by Flow cytometry
- Each lot tested to be free from infectious agents and contaminants
The line of ImmunoPure™ cells includes the following:
- PBMCs
- CD14+ Monocytes
- CD19+ B Cells
- CD4+ Helper T Cells
- Dendritic Cells
- CD8+ Cytotoxic T Cells
- CD56+ Natural Killer (NK) Cells
Find More Primary Cells from AMSBIO
Figure 1: Third Party Fresh vs Thawed Viability Test
- PBMCs: these human peripheral blood cells consist of the lymphocyte and monocyte fractions of whole blood. The mononuclear cells derived from adult peripheral blood
contain large numbers of mature immune cells.
- CD14+ Monocytes: are immature phagocytic cells that circulate in blood, phagocytize and degrade microbes and particulate matter. Once inside tissues, monocytes
mature into macrophages.
- CD19+ B Cells: are humoral immune response lymphocytes, with the primary duty of making antibodies against antigens, perform the role of antigen-presenting cells
(APCs), and eventually developing into memory B cells after activation.
- CD4+ Helper T Cells: play a crucial role in establishing and maximizing the capabilities of the immune system. These cells are unusual in that they have no cytotoxic
or phagocytic activity; they cannot kill infected host cells or pathogens. CD4+ T cells are essential in determining B cell antibody class switching, in the activation
and growth of cytotoxic T cells, and in maximizing bactericidal activity of phagocytes such as macrophages.
- Dendritic Cells: their main function is to process antigen material and present it on the surface to other cells of the immune system. They act as messengers between
the innate and adaptive immune systems. Once activated, dendritic cells migrate to the lymphoid node where they interact with T cells and B cells to initiate and shape
the adaptive immune response.
- CD8+ Cytotoxic T Cells: a sub-group of T lymphocytes that are capable of inducing the death of infected somatic or tumor cells. They are recognized as Cytotoxic
T cells once they become activated and are generally classified as having a pre-defined cytotoxic role within the immune system.
- CD56+ Natural Killer (NK) Cells: these cytotoxic lymphocytes constitute a major component of the innate immune system and are able to kill target cells without
prior exposure. NK cells are able to decipher between self and target cells then release small cytoplasmic granules of perforin and granzyme proteins to induce
programmed target cell death.
The lymphocyte and monocyte fractions are isolated from whole blood using apheresis, a technology in which the blood of a donor
or patient is passed through an apparatus that separates out these fractions, and returns the remainder to the donor's circulation. After collection, the cells are
treated with Ficoll® in order to deplete granulocytes, and red blood cells. These PBMCs are immediately cryopreserved by decreasing the temperature by 1 degree
Celsius/minute down to -170oC and using proprietary reagents to ensure high survival and recovery rates. The ImmunoPure™ cells are high quality products and research
tools that represent a diverse and heterogeneous population of donors.
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