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The Highest Efficiency Electrocompetent Cells Ever!
- CloneCatcher™ Gold with a guaranteed minimum efficiency between 8 x 1010 and 1.2 x 1011*
- Construct libraries of greater complexity than ever before (see Fig. 1)
- Library construction possible with small amounts of input DNA
- Use less topoisomerase-loaded vector and reduce overall cost
- Convenient single-use-tubes
- Super-fast 10 minute protocol
Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries.
The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli. Electroporation is one
method to efficiently introduce DNA into E. coli.
CloneCatcher™ Gold are based on a proprietary method
that allows rapid development and testing of new strains that are task specific. The resulting mutants have optimized performance profiles that produced substantially enhanced results.
The proof is on the plates! (Click on image to enlarge)
Figure 1: CloneCatcher™ DH5G Gold Electrocompetent E. coli versus major competitors. One ul of T4-ligated-DNA
library electroporated into CloneCatcher™ DH5G cells and competitor cells following each manufacturers instructions:
Plate 1. ElectroTen-Blue from Agilent
Plate 2. MegaX DH10B™ from Life Technologies
Plate 3. NEB 5-alpha from New England BioLabs
Plate 4. E. cloni® 10G SUPREME from Lucigen
Plate 5 and 6. CloneCatcher™ Gold DH5G Electrocompetent E. coli
AMSBIO is pleased to introduce the highest performing electrocompetent bacterial cells ever created: the CloneCatcher™ DH5G Electrocompent E. coli.
CloneCatcher™ exhibits extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 1011 cfu/ug pUC19 DNA.
These cells meet the needs of scientists that are seeking to find rare clones or are building complex or metagenomic libraries. CloneCatcher™ DH5G siqnificantly outperforms competitors when
introducing ligated DNA. When input DNA is limiting, CloneCatcher™ DH5G enhances the likelihood of success.
All CloneCatcher™ Electrocompetent cells are provided in a convenient single use package so efficiency-robbing freeze-thaw cycles are eliminated, and users can get the best
possible results every time. Win the race to discover new clones by ordering your CloneCatcher™ cells today.
Relative efficency of electroporations using T4-ligated DNA library(Click on image to enlarge)
Figure 2: A plasmid library was prepared using 100 ng of vector, a 4x molar ratio of insert, and T4 ligase. The reaction was incubated
overnight at 15oC, then purified using a Qiaquick PCR purification kit. One ul of the ligation mix was electroporated into CloneCatcher™ DH5G and competitors cells following manufacturers
conditions. 25ul of the final 1ml recovery volume were plated on LB carbenicillin plates and incubated at 37oC overnight. Relative efficiencies were calculated based on the average number of
clones produced on duplicate plates.
Contents
CloneCatcher™ Gold DH5G Electrocompetent E. coli: 10 x 20ul. Plating Medium: 2 x 6.0 ml. pUC19 Positive Control Plasmid: 20.0ul (10 pg/ul)
Inquire for bulk amounts and custom packaging. Shipping and Storage CloneCatcher™ Electrocompetent cells are shipped frozen on dry ice. For maximum product performance, store
cells at -80oC upon receipt, and avoid multiple freeze-thaw cycles before use.
* Patents pending
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