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TRU Block, the most powerful active blocker on the market today, actively blocks Heterophilic Antibody (HA) interference in immunoassays including, but not limited to, Human Anti-Mouse Antibody (HAMA) interference. Now, TRU Block outperforms a competitor's active blocker when tested against samples containing Rheumatoid Factor. |
Seven different HAMA/RF samples were tested at varying concentrations and in two commercial ELISA assays. In all testing of HAMA/RF samples with blockers being added to sample diluent buffer, TRU Block performed equal to or better than the competitor's active blocker.
Illustrated in the graph below is TRU Block performance when tested with Rheumatoid Factor (RF) Control Serum(Cat.# A12916H). The control serum created a false positive interference signal in a commercial CA19-9 Immunoassay. The interference could not be reduced by Mouse IgG or Rabbit IgG. In this case, only the active blockers were effective.
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What is Heterophilic Antibody Interference? |
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- Heterophilic Antibodies are a group of human antibodies against animal IgG. HA interference is a well known interference factor in diagnostic assays, often causing false positive results.
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- The most known Heterophilic Antibody interference is HAMA. HA to other animals such as Goat (HAGA), Sheep (HASA), Rabbit (HARA), etc. may also cause false results.
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- The frequency of such interference is low, but the false results have significant impact on the quality and competitiveness of the diagnostic assays as well as on the lives of those individuals who have been falsely diagnosed.
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What is the difference between TRU Block and Mouse IgG? |
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