amsbio supplies Gold-in-a-Box™
an easy-to-use kit for preparing highly reactive gold conjugates.
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The Gold-in-a-Box™ kit enables users to quickly determine (in less than
one hour) the optimal colloidal-gold binding conditions for attaching soluble proteins, and the Fc portion of purified monoclonal and polyclonal
antibodies. The resulting gold particles are highly active and greatly increase the sensitivity of lateral-flow tests. Customized components are available upon request.
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Pictured above microwell-ELISA-like sensitivity
on a rapid, lateral-flow platform. Pictured is an E. coli 0157:H7 assay that achieves an impressive 10^3 level of sensitivity. Traditional
rapid assays have a detection limit of 1-5 ng/ml. We have demonstrated that our patented C-FLAT® assay platform will permit detection levels
of 50 to 250 pg/ml.
Download further info on collodial gold conjugation
The Bioassay Works range is based on gold nanoparticles so small they appear intensely red and remain suspended in water making them ideal for rapid, point of care (or POC) assays to detect minute traces of important substances.
To accomplish this feat, nano-gold particles are coated with appropriate receptors that react specifically with the molecules to be detected.
Further information of available gold solutions, pre-conjugated gold solutions and assays can viewed in our online brochure:
To view all available Colloidal Gold Solutions
Pictured above is the titrated gold sol and the evaluation antibody. The test demonstrates the BioAssay Works buffer determining
optimal-coating conditions for antibody or protein. As seen in the photo, the combination of the gold sol, the buffers and the
addition of the antibody of interest produces a reaction indicating the isoelectric point of the antibody or protein of interest.
Background:
Lateral-flow chromatographic and flow-through tests offer
fast detection of critical components for use in point-of-care testing. The key to
flow-through tests is the ability to covalently attach antibodies to intensely colored, nanometer particles.
Gold sols that bind ligands through a sulfur bond have proved highly
successful for this application. For optimal binding of the antibody or protein while retaining a high degree of specific activity, the pH of
the gold sol must be adjusted to slightly above the iso-electric point of the coating antibody or protein.
This is done through a series of pH titrations with the provided buffers.
Varying amounts of buffers A and B, and varying amounts of buffers C and D are added to the gold to create a pH 5-11 range. Next, antibody or
protein is added, and after 30 minutes the reaction is stopped.
Lateral-flow chromatographic and flow-through tests
offer fast detection of critical components for use in point-of-care testing.
The key to flow-through tests is the ability to covalently attach antibodies to intensely colored, nanometer particles.
Gold sols that bind ligands through a sulfur bond have proved highly
successful for this application. For optimal binding of the antibody or protein while retaining a high degree of specific activity, the pH
of the gold sol must be adjusted to slightly above the iso-electric point of the coating antibody or protein.
This is done through a series of pH titrations with the provided buffers. Varying
amounts of buffers A and B, and varying amounts of buffers C and D are added to the gold to create a pH 5-11 range. Next, antibody or
protein is added, and after 30 minutes the reaction is stopped.