Enzyme & Antibodies for Neuroscience and Glycoanalysis
Chondroitin Sulfate Proteoglycans (CSPGs) are involved in the inhibition of axon regeneration after various forms of damage to the Central Nervous System, including stroke and spinal cord injury.
The enzyme Chondroitinase ABC (purified from Proteus vulgaris) degrades these CSPGs, and has been shown to promote functional recovery and neural regeneration in addition to its role as a tool in glycoanalysis.
Chondroitinase ABC enzyme (Protease Free, BSA Free, low endotoxin)
- Catalyzes the removal of Chondroitin Sulfate and Dermatan Sulfate side chains of proteoglycans
- Highly specific for glycosaminoglycan (GAG) chains without activity on core proteins, keratan sulfate chains, and heparin/heparan sulfate chains
For use in:
- in vivo & in vitro models of CNS damage/repair (perineuronal net removal and neural regeneration)
- Glycoanalysis - structure and function of proteoglycans and glycosaminoglycans (GAGs)
- Disc degeneration and discogenic low back pain (LBP) models
AMSBIO’s new Chondroitinase ABC is a replacement for the protease-free Chondroitinase ABC (100332-1A) formerly supplied
by Seikagaku Biobusiness Corporation (and available through AMSBIO), which was discontinued by the manufacturer in 2011 as part of their broader
decision to pull out of the research reagents and glycobiology biochemicals business.
Chondroitin Sulfate “stub” antibodies
Along with our Chondroitinase ABC, we also supply three monoclonal antibodies that recognise unsulfated (0S),
& 6-sulfated (6S) Chondroitin & Dermatan Sulfate, following Chondroitinase ABC digestion of proteoglycans or perineuronal nets.
|Perineuronal Net in rat cerebellum 1B5(+). Green: 1B5(+) – Orange: Nuclear Staining.
||Epitope mapping scheme of 1B5, 2B6, and 3B3 Antibodies on Chondroitin Sulfate
Citations for Chondroitinase ABC (AMS.E1028):
Roucourt, Bart, Sofie Meeussen, Jie Bao, Pascale Zimmermann, and Guido David. (2015) Heparanase activates the syndecan-syntenin-ALIX exosome pathway. Cell research .
Xu, Chen, Michelle C. Klaw, Michel A. Lemay, Peter W. Baas, and Veronica J. Tom. (2015) Pharmacologically inhibiting kinesin-5 activity with monastrol promotes axonal regeneration following spinal cord injury. Experimental Neurology 263: 172–176
Heidemann, Martina, Jürg Streit, and Anne Tscherter (2014) Functional regeneration of intraspinal connections in a new in vitro model. Neuroscience 262: 40–52.
Milby, Andrew H. et al (2014) Percutaneous Delivery of Chondroitinase ABC Induces Moderate Disc Degeneration in a Goat Model. University of Pennsylvania Orthopaedic Journal 74: 173-5.
Nelson, A., Berkestedt, I., & Bodelsson, M. (2014) Circulating glycosaminoglycan species in septic shock. Acta Anaesthesiologica Scandinavica. 58 (1): 36–43.
Silver et al (2013) Chondroitin Sulfate Proteoglycans Potently Inhibit Invasion and Serve as a Central Organizer of the Brain Tumor Microenvironment. Journal of Neuroscience 33(39): 15603-15617.
Lensjo, K.K., Dick, G., Norheim, E. S., Hafting, T., and Fyhn, M. (2013) Cortical processing in awake, behaving rats after removal of perineuronal nets. Poster exhibited at SfN Neuroscience 2013 Conference.
Background / Reviews:
Burnside, E. R., & Bradbury, E. J. (2014) Review: Manipulating the extracellular matrix and its role in brain and spinal cord plasticity and repair. Neuropathology and applied neurobiology, 40(1), 26-59.
Caterson, B. (2012) Fell‐Muir Lecture: Chondroitin sulphate glycosaminoglycans: fun for some and confusion for others. International journal of experimental pathology, 93(1), 1-10.
Fox, K., & Caterson, B. (2002) Freeing the brain from the perineuronal net. Science, 298 (5596), 1187-1189.
Highly cited papers featuring Seikagaku Chondroitinase ABC
Bradbury, E. J., Moon, L. D., Popat, R. J., King, V. R., Bennett, G. S., Patel, P. N., & McMahon, S. B. (2002) Chondroitinase ABC promotes functional recovery after spinal cord injury. Nature, 416(6881), 636-640.
Moon, L.D.F., Asher, R.A., Rhodes, K.E., & Fawcett, J.W. (2001) Regeneration of CNS axons back to their target following treatment of adult rat brain with chondroitinase ABC. Nature Neuroscience 4, 465-466.
Thuret, S., Moon, L.D.F., & Gage, F.H. (2006) Therapeutic strategies for SCI. Nature Reviews Neuroscience 7, 628-643.