| Conditions |
|
| Volume of Cellufine Sulfate:1ml （ID 8×20 mm) |
| Liner Velocity: |
120cm/hr |
| Equilibration buffer: |
0.01M Phosphate buffer, pH 7.4 |
| Washing buffer: |
0.01M Phosphate buffer, 0.19M NaCl, pH7.2 |
| Elution buffer: |
0.01M Phosphate buffer, 3M NaCl, pH7.0 |
| Virus Strain: |
A/duck/Hokkaido/Vac-1/04 (H5N1) β-Propiolactone |
| --- |
Allantoic Fluid was filtered after centrifuged |
| (Centrifugation: 8,000g×30min, Filter:0.45μm Cellulose Acetate) |
| Sample Volume: |
6 ml : (The virus sample from professor Kida.(Hokkaido Univ. Graduate school of veterinary medicine. ) |