4C5 Anti-DDK Monoclonal Antibody
4C5 anti-DDK antibody for the detection of DYKDDDDK epitope (same epitope as Flag®)
- Validated for Western blot, immunoprecipitation, immunofluorescence and flow cytometry
- Higher sensitivity and specificity compared to other vendors anti-flag antibodies
- Purify recombinant proteins with high purities and yields
- Detect recombinant proteins with low expression levels
4C5 anti-DDK antibody has already been used to:
- Validate the recombinant protein expression from more than 15,000 TrueORF
cDNA clones and VERIFY™ overexpression lysates
- Purify more than 5,000 human recombinant proteins from HEK293 cells
- Purify immunogens under native conditions for the development of TrueMAB antibodies
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Immunoblot analysis of 7 randomly picked VERIFY™ myc-DDK tagged overexpression
lysates and myc-DDK tagged Western molecular weight marker (MWM1001) using 4C5 anti-DDK
antibody at 1:2000 dilution
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| Code |
Description |
Data Sheet |
Price |
| TA50011-100 |
4C5, Anti-DDK monoclonal antibody (100 ul) |
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| TA50011-1 |
4C5, Anti-DDK monoclonal antibody (1 ml) |
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| TA50011-5 |
4C5, Anti-DDK monoclonal antibody (5 ml) |
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Hela cells transiently transfected with myc-DDK tagged GFAP ORF cDNA clone were immunostained with anti-DDK (1:1000)and stained with Alexa-408 conjugated secondary antibody (1:2000). The nuclei (Blue) were counter-stained with DAPI. |
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HEK293T cells transfected with either myc-DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (Blue) were immunostained with anti-DDK antibody and analyzed by flow ctyometry. |
For bulk antibody package, please contact Tech Support
Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a trademark of Sigma-Aldrich.