The DDK-tag is included in the pCMV6-Entry vector that forms the standard backbone of the TrueORF cDNA series.

• Validated for Western blot, immunoprecipitation, immunofluorescence and flow cytometry
• Higher sensitivity and specificity compared to other vendors anti-flag antibodies
• Purify recombinant proteins with high purities and yields
• Detect recombinant proteins with low expression levels

4C5 anti-DDK antibody has already been used to:

• Validate the recombinant protein expression from more than 15,000 TrueORF cDNA clones and VERIFY(tm) overexpression lysates
• Purify more than 5,000 human recombinant proteins from HEK293 cells
• Purify immunogens under native conditions for the development of TrueMAB antibodies

Immunoblot analysis of 7 randomly picked VERIFY(tm) myc-DDK tagged overexpression lysates and myc-DDK tagged Western molecular weight marker (MWM1001) using 4C5 anti-DDK antibody at 1:2000 dilution

Hela cells transiently transfected with myc-DDK tagged GFAP ORF cDNA clone were immunostained with anti-DDK (1:1000)and stained with Alexa-408 conjugated secondary antibody (1:2000). The nuclei (Blue) were counter-stained with DAPI.

HEK293T cells transfected with either myc-DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (Blue) were immunostained with anti-DDK antibody and analyzed by flow ctyometry.